The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri
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منابع مشابه
The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri
BACKGROUND The multicellular green alga Volvox carteri represents an attractive model system to study various aspects of multicellularity like cellular differentiation, morphogenesis, epithelial folding and ECM biogenesis. However, functional and molecular analyses of such processes require a wide array of molecular tools for genetic engineering. So far there are only a limited number of molecu...
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The study of the dependence of reactions on the concentration of various compounds is often of great value in determining reaction mechanisms . In chemical kinetics, it is often found that, according to van't Hoff's rule, the order of dependence of a reaction on the concentration of some compound involved in that reaction is equal to the number of molecules of the compound involved in each reac...
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Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
متن کاملAn inducible arylsulfatase of Volvox carteri with properties suitable for a reporter-gene system. Purification, characterization and molecular cloning.
The multicellular green flagellate Volvox carteri synthesizes a periplasmic arylsulfatase in response to sulfur deprivation. The inducible enzyme has been purified to homogeneity and characterized. The corresponding gene and cDNA have been cloned. Determination of the sequence of genomic clones and comparisons to the cDNA sequence, revealed sixteen introns and seventeen exons that encode a 649-...
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A preliminary genetic analysis of a number of genetic variants of Volvox carteri f. nagariensis is presented. Techniques are outlined for mutagenesis of Volvox, isolation of mutants and routine genetic analysis. All of the mutants show simple Mendelian segregation patterns and have been tentatively placed in 14 linkage groups.
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2015
ISSN: 1472-6750
DOI: 10.1186/s12896-015-0122-3